Table 3 Kinetic parameters of CapGH3a and their respective orthologs

From: A functionally augmented carbohydrate utilization locus from herbivore gut microbiota fueled by dietary β-glucans

Substrate

CapGH3a (this work)

CapGH3a W88A (this work)

BACOVA_0273815

kcat (s−1)

Km*

kcat/Km#

kcat (s−1)

Km*

kcat/Km #

kcat (s−1)

Km*

kcat/Km #

Laminarin (Eisenia bicyclis)a

3.1 ± 0.1

3.3 ± 0.1

0.95

0.004 ± 3.0

1.9 ± 0.3

0.002

N.t.

N.t.

-

Laminarin (Laminaria digitata)b

1.6 ± 0.1

3.6 ± 0.3

0.44

0.003 ± 3.9

4.6 ± 0.7

0.0007

N.t.

N.t.

-

β-glucan (from Oat)c

N.d.

N.d.

-

N.d.

N.d.

-

N.t.

N.t.

-

β-glucan (from Yeast)d

N.d.

N.d.

-

N.d.

N.d.

-

N.t.

N.t.

-

pNp-β-Glucopyranoside

14.8 ± 0.4

8.9 ± 0.6

1.67

1.31 ± 0.1

3.6 ± 0.5

0.37

0.2 ± 0.01

2.5 ± 0.1

0.08

Cellobiose (G4G)

6.3 ± 0.3

8.9 ± 1.1

0.71

0.01 ± 0.0

47.9 ± 4.5

0.0003

N.t.

N.t.

-

Laminaribiose (G3G)

6.1 ± 0.3

6.7 ± 0.6

0.91

0.17 ± 0.0

61.2 ± 9.0

0.003

N.t.

N.t.

-

Gentiobiose (G6G)

30.5 ± 0.6

4.7 ± 0.3

6.5

0.34 ± 0.0

27.7 ± 3.8

0.012

N.t.

N.t.

-

  1. *Km is represented in mM for pNp-β-Glucopyranoside and oligosaccharides and in mg mL−1 for polysaccharides.
  2. #kcat/Km is represented in s−1 mM −1 for pNp-β-Glucopyranoside and oligosaccharides and in s−1 mg−1 mL for polysaccharides.
  3. N.t. not tested, N.d. not detected.
  4. Linkage type: a–β(1,3)-glucan with low β(1–6) substitution [7:1 molar ratio]; b–β(1,3)-glucan with high β(1–6) substitution [3:2 molar ratio]; c–β(1–3)/(1,4)-glucan; and d–β(1,3)-glucan containing long β(1,3)-glucan branches extended from β(1,6)-linked branch points.
Back to article page