Fig. 8: AI-2 promotes ΔM biofilm formation.
From: Mechanisms of S. agalactiae promoting G. vaginalis biofilm formation leading to recurrence of BV
A Dual-species 48-hours biofilm status as observed under CLSM; a1-3 represent ΔM, a4-6 represent ΔM + 7.6μm AI-2 (100× magnification). B Crystal violet quantification of biofilm with ΔM and added gradient AI-2 (from 7.6 nM to 7.6 µM). C Dual-species 48-hours biofilm observed under SEM; c1-3 represent ΔM; c4-6 represent ΔM + 7.6 µM AI-2. Data are shown as mean ± SEM (n = 9). Significant differences with p values determined using Student’s t-test and adjusted for the false discovery rate, are marked by * for p value < 0.05, ** for p value < 0.01 and *** for p value < 0.001. D RT-qPCR results of biofilm-related genes after adding exogenous AI-2 to GV. Data are shown as mean ± SEM (n = 3).
