Fig. 5: In vitro effects of 3,4-dihydroxybenzoic acid (3,4-DHBA).
From: Gut microbes modulate the effects of the flavonoid quercetin on atherosclerosis
A 3,4-DHBA does not affect LPS-mediated activation of inflammation in macrophages. B The detrimental effect of LPS on endothelial cells monolayer integrity is ameliorated by 3,4-DHBA. Human aortic endothelial cells were grown to confluence on transwell inserts (12 well plates) and exposed to LPS (100 ng/ml) and LPS together with two different BA concentrations for 24 h. After that time, the endothelial monolayer integrity was evaluated using a voltohmmeter. Cells were equilibrated and transendothelial electrical resistance (TEER) was measured at room temperature. Data are presented as mean ± SEM (n = 3). LPS lipopolysaccharide, 3,4-DHBA 3,4-dihydroxybenzoic acid.
