Fig. 3: Deficiency of the tnaA gene impaired P. gingivalis growth, virulence factor expression and biofilm formation. | npj Biofilms and Microbiomes

Fig. 3: Deficiency of the tnaA gene impaired P. gingivalis growth, virulence factor expression and biofilm formation.

From: Regulation of tryptophan-indole metabolic pathway in Porphyromonas gingivalis virulence and microbiota dysbiosis in periodontitis

Fig. 3

a Indole assay of the 24 h-culture of WT and ΔtnaA supplemented with tryptophan (3 mM). The 24 h-culture of P. gingivalis grown in blank medium was used as control. b Colony formation of WT and ΔtnaA on the blood agar plates (7 days). c Growth curve of WT and ΔtnaA. d Differential proteins of WT and ΔtnaA after 24-h cultivation with tryptophan (3 mM) identified by proteomics. e–i qRT-PCR measurement of the tnaA and P. gingivalis virulence factors including luxS (f), kgp (g), fimA (h) and rgp (i) expression of WT and ΔtnaA. j Biofilm assay of WT under 24-h indole stimulation. k Biofilm assay of WT and ΔtnaA under 24-h tryptophan stimulation. l Biofilm assay of ΔtnaA supplemented with exogenous indole for 24 h. Statistical significance was determined using Student’s t test and one-way ANOVA followed by Dunnett’s multiple comparisons test. *p < 0.05, **p < 0.01, ***p < 0.001.

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