Fig. 1: Expression of Mn oxidase gene promoters in the presence and absence of Mn(II) in microcolonies of P. putida GB-1. | npj Biofilms and Microbiomes

Fig. 1: Expression of Mn oxidase gene promoters in the presence and absence of Mn(II) in microcolonies of P. putida GB-1.

From: Population-level control of two manganese oxidases expands the niche for bacterial manganese biomineralization

Fig. 1

a, b Violin plot of the fluorescence intensity distribution in microcolonies of wild-type (WT) and bioreporter (PmnxG, a; PmcoA, b) strains without Mn and with 50 µM Mn(II), where n is the number of microcolonies analyzed for each condition. The eCherry fluorescent signal represents the pixel intensity distribution within the boundaries of the microcolonies in the phase contrast images. The fluorescence threshold is set as the 99th quantile of the wild-type distribution, calculated using background-subtracted images. Outliers removed at 6× the SD of the respective distributions. c, d Representative phase contrast and fluorescence images of 48-h-grown P. putida GB-1 PmnxG (c) and PmcoA (d) microcolonies grown in the absence of Mn (top panels) and in the presence of 50 µM Mn(II) (bottom panels). Values on the fluorescence micrographs represent the range of lowest to highest pixel fluorescence signal intensity in the displayed image.

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