Fig. 2: EF-Tu is the adhesion protein functioning with the “Barrel-like adhesion domain”.
From: Elongation factor Tu promotes the onset of periodontitis through mediating bacteria adhesion
a, b Immunotransmission electron microscopy images to characterize the cellular localization of EF-Tu in the planktonic and biofilm states of S. oralis. The purple area enclosed by the white dashed box indicates the bacterial membrane. The red arrow points to EF-Tu (scale bar, 100 nm). c Quantification analysis of EF-Tu location in images (n = 6). d The MSD versus time interval of BSA and EF-Tu. α value is shown along the curve. e The MSD versus time interval of three groups of S. oralis. α value is shown along the curve. f The step-size distribution of S. oralis at Δt = 0.22 s. g The MSD versus time interval of S. oralis. h Molecular dynamics simulation of EF-Tu binding to Fn. Red represents EF-Tu, and silver represents Fn. i Molecular dynamics simulation of EF-Tu adhering to the HA plane. Red represents EF-Tu, and silver represents the HA plane. j, k The potential energy of amino acid residues in the ED3 domain. The red bar represents the adhesive amino acid residues in both simulated systems. l The “Barrel-like adhesion domain” consists of the adhesive amino acid residues in both simulated systems. The silver represents EF-Tu, the yellow represents the “Barrel-like adhesion domain”, and the red represents the ED3 domain. m The MSD versus time interval of eleven groups of EF-Tu proteins. n Step-size distribution of EF-Tu in protein groups at Δt = 50 ms. o The MSD versus time interval of eleven groups of S. oralis. p Step-size distribution of EF-Tu in S. oralis groups at Δt = 0.22 s. Data information: In c, bars and error bars represent mean ± SD. Data were analyzed using Two-way ANOVA. ns means no significance, *P < 0.05, **P < 0.01, ***P < 0.001.
