Fig. 2: Validation of taxonomic resolution and richness of 16S rRNA sequencing reads using mock community datasets.
From: Refining microbiome diversity analysis by concatenating and integrating dual 16S rRNA amplicon reads
a The comparison of the mapped reads ratio from the SRP291583 dataset. ME: the method merging raw paired-end reads, DJ: concatenating raw paired-end reads. b Alpha diversity metrics across methods, including observed species and Shannon index. c NMDS plot based on Jaccard distance across the analytical methods. d Family-level relative abundance, with “Others” indicating taxa below 1% relative abundance. The relative abundance for the theoretical composition of ZIEL-II mock community24 is indicated by an asterisk (*). e The distribution of difference values between theoretical and actual values for each family. f Comparison of relative abundance between theoretical values (T.V.) and specific 16S rRNA regions from ZIEL-2 mock datasets. g Evaluation of precision, recall, and F-measure for the ME and DJ pipelines using the V1-V3 and V6-V8 regions, based on the SILVA DB.
