Fig. 2: Dysbiotic microbes in vaginal and rectal microbiomes enriched in endometrial cancer patients compared to benign.

Limosilactobacillus is depleted, whereas Anaerococcus, Prevotella, Porphyromonas, and Peptoniphilus are enriched in EC samples when compared to benign conditions in vaginal samples. Buttiauxiella is enriched in rectal samples of patients with EC while depleted in Peptoniphilus and Prevotella in rectal samples. Enrichment in bacterial taxa among EC compared to benign conditions prior (A) and after adjusting for BMI and menopausal status (B). Comparison of differentially abundant taxa of EEC vs. Other ECs with qvalue of < 0.05. Dots in dark orange indicate >1-Fold change. The bacterial enrichment was performed utilizing ANCOM-BC, visualized where taxa with at least 0.7 LFC and other significant taxa are in the supplement. The threshold for LFC of 1 or greater (orange), below 1 (gray), and all p values were Bonferroni false discovery adjusted. C Boxplots of taxa indicated as significant differentially abundant log10 transformed in this cohort, where the disease group observed abundance. Light blue indicates benign, light purple indicates hyperplasia, light orange indicates EEC and dark orange indicates Other ECs. The Kruskal-Wallis test performed additional abundance differences where * is <0.05, ** is <0.01, *** <0.001, and **** <0.0001 p value.