Fig. 5: Fusobacterium nucleatum subspecies animalis abundance by clade and correlated with FISH.
From: Fusobacterium nucleatum is enriched in invasive biofilms in colorectal cancer
A Relative abundance by 16S rRNA amplicon sequencing of Fusobacterium nucleatum subspecies animalis clade 1 (Fna C1) and clade 2 (Fna C2) in pairs of tumor (blue circle) and normal (clear circle). Tumor/normal pairs analyzed by two-tailed Wilcoxon matched-pairs signed rank test, and tumor/tumor or normal/normal comparisons analyzed by two-tailed Mann Whitney test, with p < 0.05 considered significant. B Relative abundance of Fna C1 and C2 stratified by biofilm (BF) status and C, D by both biofilm status and tumor stage. Bars indicated median. Of 112 individuals with CRC in the study, 108 tumor and normal pairs were available for 16S rRNA sequencing analysis. Numbers below each graph depict the number of samples out of the total in which each clade was not detected by sequencing. E Heatmap depicting relative abundance by 16S rRNA amplicon sequencing for the Fusobacterium genus and each labeled Fusobacterium species or clade for n = 29 tumors where dense blooms of Fusobacteria were visualized by FISH.
