Fig. 6: ACh supplementation restores the composition of gut microbiota and SCFAs production.
From: TSP50 deficiency in neural stem cells aggravates colitis in mice by altering intestinal microbiome
a ELISA analysis of ACh levels in the colon after ACh supplementation (TSP50fl/fl, n = 6; TSP50fl/flNestinCre, n = 6; ACh-TSP50fl/fl, n = 8; ACh-TSP50fl/flNestinCre, n = 8). b PCoA analysis of fecal microbiota using weighted UniFrac distances of beta diversity after ACh supplementation. c Histogram of phylum-level species composition after ACh supplementation. d Histogram of genus-level species composition after ACh supplementation. e Histogram of species difference comparison for the top 10 abundances at phylum-level (n = 6). f Histogram of species difference comparison for the top 10 abundances at genus-level (n = 6). g Heat map of the relative abundance of the dominant genus. h LEfSe analysis of differential microbiota in TSP50fl/fl mice before and after ACh treatment. i LEfSe analysis of differential microbiota in TSP50fl/flNestinCre mice before and after ACh treatment. j GC-MS analysis of fecal SCFAs after ACh supplementation (n = 5). k ELISA analysis of ACh levels in the colon after ABX treatment (n = 6). l ELISA analysis of ACh levels in the colon after co-housing treatment (n = 6). m ELISA analysis of ACh levels in the colon after FMT and AKK replantation (n = 6). Values were expressed as means ± SEM. One‑way ANOVA was used to analyze (a, j, k, l, m), Kruskal-Wallis H test was used to analyze (e, f). ns (no significance), * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
