Fig. 2: DEHP disrupted the HPO axis regulatory homeostasis.

A Representative graphs illustrating the immunofluorescence of GnRH in various DEHP treatment groups and control groups. Scale bars: 50 µm. B Representative graphs demonstrating immunohistochemical analysis. Scale bars: 100 µm. C Statistical plots illustrating the immunofluorescence of GnRH in various DEHP treatment groups and control groups (n = 6, Student’s t test, ***p < 0.001). D Bar chart displaying the alterations in GnRH, FSH, estradiol, and progesterone protein levels in the serum of different treatment groups and control groups (n = 6, Student’s t test, **p < 0.01; ***p < 0.001).