Fig. 2: MycoMarT7 transposon library screen reveals mutants with altered eDNA levels in biofilm-forming culture conditions. | npj Biofilms and Microbiomes

Fig. 2: MycoMarT7 transposon library screen reveals mutants with altered eDNA levels in biofilm-forming culture conditions.

From: M.marinum lacking epsH shows increased biofilm formation in vitro and boosted antibiotic tolerance in zebrafish

Fig. 2

a GelRed-based measurement of eDNA levels in biofilm and planktonic M. marinum cultures revealed higher eDNA content in the biofilm. b Normalized fluorescence values of single clones grown in biofilm forming conditions after GelRed staining. Values were normalized to the average value of the plate. The clones having highest and lowest 1% of fluorescence were selected for further analysis. c Secondary screen of top and bottom 1% of eDNA abundancy clones by AccuBlue staining confirms the altered eDNA levels. One-way ANOVA followed by Dunnett's multiple comparison test. d The fluorescence signal of DNA binding dye AccuBlue increases by time in biofilm forming conditions and is significantly higher in epsH mutant strain than in WT. DNase treatment significantly reduces the fluorescence signal. The colour of the asterisk indicates the condition which the statistical analysis is compared to. Two-way ANOVA followed by Bonferroni’s multiple comparison test. P-values: * < 0.05, ** < 0.01, *** < 0.001.

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