Table 2 Primers for real-time quantitative PCR used in the present study
From: Lactobacillus regulate muscle fiber type conversion in Chinese native pigs via tryptophan metabolism
Gene names | Primers | Sequences (5′−3′) | Product size (bp) |
|---|---|---|---|
Gene primers for pig | |||
AMPK | Forward | TTCGGCAAAGTGAAGGTTGG | 224 |
Reverse | AGCTCGCCTCCTGAAACATA | ||
GAPDH | Forward | GGAGCGAGATCCCGCCAACA | 158 |
Reverse | ACATGGGGGCATCGGCAGAA | ||
IDO1 | Forward | CTCCGTCCGTGAGTTTGTTC | 174 |
Reverse | GAAGGCTCTTCGGATGCTTG | ||
KYAT1 | Forward | AGGGCAGCTACTTCCTCATC | 250 |
Reverse | CACTTCTGCAGCTTCTCGTC | ||
KYAT2 | Forward | AGATGCAAAGAACCCCGAGA | 181 |
Reverse | GGGCTTGTTGAACTGCAGAA | ||
KYAT3 | Forward | GGACAGCAGCGAACCTTATG | 166 |
Reverse | AGCAGCATCCAGTGTAGTGT | ||
KYAT4 | Forward | GTAATTCTCCTGCACGCCTG | 155 |
Reverse | TCCTTGTTACCATCGCCACT | ||
MyHC I | Forward | GGCCCCTTCCAGCTTGA | 63 |
Reverse | TGGCTGCGCCTTGGTTT | ||
MyHC IIa | Forward | TTAAAAAGCTCCAAGAACTGTTTCA | 100 |
Reverse | CCATTTCCTGGTCGGAACTC | ||
MyHC IIb | Forward | CACTTTAAGTAGTTGTCTGCCTTGAG | 80 |
Reverse | GGCAGCAGGGCACTAGATGT | ||
MyHC IIx | Forward | AGCTTCAAGTTCTGCCCCACT | 76 |
Reverse | GGCTGCGGGTTATTGATGG | ||
PGC-1α | Forward | GCCCAGTCTGCGGCTATTT | 265 |
Reverse | GTTCAGCTCGGCTCGGATTT | ||
Gene primers for mice | |||
AMPK | Forward | CTCAACCGGCAGAAGATTCG | 212 |
Reverse | CGGCTTTCCTTTTCGTCCAA | ||
GAPDH | Forward | GGGTCCCAGCTTAGGTTCAT | 248 |
Reverse | CATTCTCGGCCTTGACTGTG | ||
IDO1 | Forward | TCTCAGAGAAGTTGGGCCTG | 189 |
Reverse | CAGGAGAAGCTGCGATTTCC | ||
KYAT1 | Forward | GGCAGCTACTTCCTCATTGC | 169 |
Reverse | CCTTATGATGGGGCTGGCTA | ||
KYAT2 | Forward | CCCATCAGAACTACAGCGGA | 163 |
Reverse | GGGCCCTTTTGATCAAGTCG | ||
KYAT3 | Forward | TCATTGCTGACCTTTGCGTC | 152 |
Reverse | TGTCTTGCCAGCACTTCCTA | ||
KYAT4 | Forward | CAGCCGAGATGTCTTTCTGC | 167 |
Reverse | GGACACTCTGCTCTGGGATT | ||
MyHC I | Forward | AGACGGTGACTGTGAAGGAG | 202 |
Reverse | TACACAGGCAGCCACTTGTA | ||
MyHC IIa | Forward | CAGTGTCTAAGGCCAAGGGA | 171 |
Reverse | TCTCATCAAGCTGCCTGGAA | ||
MyHC IIb | Forward | TTGAAAAGACGAAGCAGCGAC | 190 |
Reverse | AGAGAGCGGGACTCCTTCTG | ||
MyHC IIx | Forward | GCGAATCGAGGCTCAGAACAA | 138 |
Reverse | GTAGTTCCGCCTTCGGTCTTG | ||
PGC-1α | Forward | AGCCTCTTTGCCCAGATCTT | 241 |
Reverse | GGCAATCCGTCTTCATCCAC | ||
