Fig. 8: Lactobacillus amylovorus BEV-derived oleic acid mitigates oxidative stress-induced ferroptosis and enhances milk fat synthesis in mammary epithelial cells. | npj Biofilms and Microbiomes

Fig. 8: Lactobacillus amylovorus BEV-derived oleic acid mitigates oxidative stress-induced ferroptosis and enhances milk fat synthesis in mammary epithelial cells.

From: Lactobacillus amylovorus extracellular vesicles mitigate mammary gland ferroptosis via the gut-mammary gland axis

Fig. 8

a Content and composition of various fatty acids, including monounsaturated fatty acids, in Lactobacillus amylovorus extracellular vesicles. Content and composition of various fatty acids in Lactobacillus salivarius (b), Lactobacillus reuteri (c), Lactobacillus johnsonii (d), and Lactobacillus plantarum (e) BEVs. f Detection of intracellular reactive oxygen species (ROS) levels using ROS fluorescence assay. Scale bar: 200 μm. g Relative mean fluorescence intensity of ROS (n = 3). h Detection of intracellular lipid peroxidation (LPO) levels using LPO fluorescence assay. Scale bar: 200 μm. i Relative mean fluorescence intensity of LPO (n = 3). Triglyceride concentrations in the medium (j) and within cells (k) (n = 6). l Oil Red O staining of HC11. Scale bar: 100 μm. m Quantitative analysis of Oil Red O staining (n = 3). *P < 0.05; **P < 0.01; ***P < 0.001; ns not significant (P > 0.05).

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