Fig. 3: Influence of host DNA depletion on the fidelity of microbiome profiling. | npj Biofilms and Microbiomes

Fig. 3: Influence of host DNA depletion on the fidelity of microbiome profiling.

From: Benefits and challenges of host depletion methods in profiling the upper and lower respiratory microbiome

Fig. 3

Similarity in microbial composition between R_ase and other methods, and proportion of contaminating microbial reads in total microbial reads in BALF (A) and OP (B) samples. Dots indicate median values of different methods, and error bars indicate standard deviations. JSD (Jensen-Shannon distance) was calculated excluding identified contaminating components. The gray area on the left indicates the range of JSD between technical replicates, while the gray area on the right indicates the 5th to 95th percentile range of JSD among samples from different individuals. Absolute and relative abundance changes of common species in BALF (C) and OP (D) samples. Fold changes in abundances were calculated between different methods and R_ase. Asterisks indicate significant differences in abundances between different methods and R_ase, *p.adj < 0.05, **p.adj < 0.01, ***p.adj < 0.001, Wilcoxon matched pairs tests. n = 35 for BALF and n = 34 for OP.

Back to article page