Fig. 4: Integrated evaluation of host DNA depletion methods.

Radar charts showing the performance of host DNA depletion methods on five evaluation metrics in BALF (A) and OP (B) samples. Accuracy was defined as 1 subtracted by the JSD between microbial compositions of different methods and R_ase. The contamination level was defined as 1 subtracted by the proportion of contaminants in total microbial reads. Microbial proportion and accuracy were estimated excluding exogenous contamination. Maximum-minimum measures were used for data scaling. The two methods with the best performance (largest radar area) are labeled in bold. n = 35 for BALF and n = 34 for OP.