Fig. 4: F.nucleatum induced ferroptosis in the colon of UC mice. | npj Biofilms and Microbiomes

Fig. 4: F.nucleatum induced ferroptosis in the colon of UC mice.

From: Periodontitis-associated Fusobacterium nucleatum promotes ulcerative colitis by ferroptosis-mediated gut barrier disruption

Fig. 4

A Immunohistochemical staining for GPX4, FTH1, and ACSL4 (scale: 100 μm, left; 50 μm, right). Black arrows indicated expression-positive cells. B The average optical density of GPX4, FTH1, and ACSL4 was shown on the right side (n = 6). C Western blotting analysis of GPX4, FTH1, and ACSL4 and quantification. β-actin was used as the loading control (n = 3). D RT-qPCR measured the relative mRNA levels of GPX4, FTH1, and ACSL4 (n = 5). E Iron Assay Kit determined the iron levels (n = 5). F MDA Assay Kit measured the MDA levels (n = 5). G GSH and GSSG Assay Kit measured the relative GSH/ GSSG ratio (GSH%) (n = 5). H Double immunofluorescent staining for GPX4 and cytokeratin 18 (CK18) (scale: 50 μm) (n = 3). Each dot indicated an individual mouse. All data represent the mean ± SEM. *P < 0.05, **p < 0.01, ***P < 0.001, ****p < 0.0001, by one-way or two-way ANOVA, followed by a post hoc test.

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