Fig. 2: GABA screening. | npj Biofilms and Microbiomes

Fig. 2: GABA screening.

From: A rationally designed microbial consortium modulates neurodegeneration in a Drosophila melanogaster model of Parkinson’s disease

Fig. 2

A Thin-layer chromatography of bacteria supernatants. The first two lanes correspond to the standards of GABA and monosodium glutamate (MSG). The following four lines are the culture media without bacteria. The remaining lines correspond to bacterial supernatant from monoculture growth. GABA is visualized as an intensely colored band at the same height as the GABA standard. All media was supplemented with monosodium glutamate (glut) to induce GABA production. Culture medium was mZMB unless indicated, and media abbreviations are indicated in methods. D3: B. adolescentis D3. Ba: B. adolescentis ATCC 15703. G2: L. brevis G2. L: L. plantarum JDM1. S1: P. vulgatus S1. Bt: B. thetaiotaomicron VPI 5482, and Bova: B. ovatus BEI 3_8_47FAA. B Thin-layer chromatography of co-cultures. BtM38: B. thetaiotaomicron VPI 5482 and L. paracasei M38. BovaM38: B. ovatus BEI 3_8_47FAA and L. paracasei M38. G2M38: L. brevis G2 and L. paracasei M38. Lanes are as in A indicating the carbon source (Glucose or Inulin).

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