Table 1 The identified L1-, L5- and ‘F. nucleatum’-specific markers and corresponding primers designed
Taxon | Marker | Reference gene | Annotation | Primer | sequence (5’ to 3’) | Amplicon size (bp) |
|---|---|---|---|---|---|---|
L1 | L1_125 | C7Y58_06055 | hypothetical protein | L1_125-F1 | RGTRTTGGAGTTGTTCAATATGG | 93 |
L1_125-R1 | TTCMGATAARGAATTAACTTCATTTTC | |||||
L1_125-F2 | ATGAARYTAACATTACAACAAGCTATATT | 103 | ||||
L1_125-R2 | TTTAAAGGYACTACATAAYTRTCACGAAT | |||||
L1_125-F3 | ATTCGTGAYARTTATGTAGTRCCTTTAAA | 293 | ||||
L1_125-R3 | CCATATTGAACAACTCCAAYACY | |||||
L1_746 | C7Y58_07255 | DUF4037 domain-containing protein | L1_746-F | YTAAATGARTTRGGWGGAACTTTAAA | 163 | |
L1_746-R | TTTGTRCAAATCTRTATRCYTCAGTTA | |||||
L1_820 | C7Y58_01415 | hypothetical protein | L1_820-F | GTGCAACAACWATHTCWTAYTGGTATGG | 139 | |
L1_820-R | TAYTTYTCWACATARGCYTTTCCCAT | |||||
L5 | L5_211 | C4N18_13790 | hypothetical protein | L5_211-F | TTTRAATAATAAAATTGGAGTGAGA | 285 |
L5_211-R | TCTTCTGCTAGTGTTCTAGGAAGAA | |||||
L5_962 | C4N18_14645 | PaaI family thioesterase | L5_962-F | TTATYACTYTAGCTGATACTACATGTGG | 153 | |
L5_962-R | CAAGATGCTGAGCAGTRGCAGTACA | |||||
L5_1277 | C4N18_13335 | hypothetical protein | L5_1277-F | GAGGTATGGGATAGAGAACAGCCTG | 113 | |
L5_1277-R | TCATTAATRTTYTTTACATCTTCTGGTGTT | |||||
L5_1430 | C4N18_12565 | hypothetical protein | L5_1430-F | TTTCAYTACCTTGAAGAAGGAAA | 103 | |
L5_1430-R | TTTCTTTATCTTTTCCTGTAAGCATAA | |||||
L5_1621 | C4N18_10820 | hypothetical protein | L5_1621-F | GAGAATCAATTCAGAGASTACAMATTCTAT | 143 | |
L5_1621-R | AATAAAGRTTRACAGTATCCATCACTGTA | |||||
‘F. nucleatum’ | Fn_SR | C7Y58_05970 | peptidase | Fn_SR-F | YTATCCTCAATCAAGAGTTACAA | 348 |
Fn_SR-Ra | AGRATTTTAYCRGTATTAGCATCRAT | |||||
Fn_SR-Rb | AARATTTTWCCASTATTAGCATCAAT | |||||
Fn_SR-Rc | ATAACTTTCCCACTATTAGCATCAAT |
