Fig. 3
Lgr5-expressing cells are bipotent in the embryo and lineage restricted in the adult. a–c Lgr5KI; R26LSL-Tom were injected with 4OHT at stage E17 and adult. Eight weeks later, single-cell suspensions were prepared and viable (DAPI negative) cells were analyzed for expression of the tdTomato fluorescent reporter, EpCAM, and CD49f. The percentage of cells that were tdTomato positive was measured by designating a threshold above which epithelial cells of R26WT measured zero. d Lgr5KI; R26LSL-Tom pregnant mice were injected with 4OHT at stage E17. Eight weeks later, they were harvested, fixed, sectioned, and stained for Keratin 8 (K8, blue), Keratin 14 (K14, green), and tdTomato (red). tdTomato co-staining with K8-expressing luminal cells and K14-coexpressing myoepithelial cells are indicated by the white and gray arrows, respectively
