Fig. 4

Analysis of fetal mammary rudiments heterozygous (Lgr5KI^HET) and homozygous (Lgr5KI^HOM) for the Lgr5KI allele. a Fetal mammary rudiments were isolated from Lgr5KI^HET and Lgr5KI^HOM littermates at stage E18 and stained in whole mount for GFP (top, bar represents 200 μM), and Keratin 8 (K8, green) and Keratin 14 (K14, red, bottom). b Single-cell suspensions were prepared from Lgr5KI^HET and Lgr5KI^HOM littermates at stage E18. Live, single, epithelial (EpCAM^POS) cells were analyzed for GFP expression. c Single-cell suspensions were prepared from Lgr5KI^HET and Lgr5KI^HOM littermates at stage E18. Live, single, epithelial cells were analyzed for EpCAM and CD49f expression by FACS. d Single-cell suspensions were prepared from Lgr5KI^HET and Lgr5KI^HOM littermates at stage E18. Live, single, EpCAM^HIGH; CD49f^HIGH; GFP^POS cells were sorted and plated in to 3D culture containing 2% Matrigel. After 1 week, spheres were quantified and percent SFCs was determined by the number of spheres counted per the number of cells plated. Percent SFC is reported as averages of biological replicates and error bars represent standard deviations from three separate experiments, each with at least three technical replicates