Fig. 5
Lgr5 function is not required for mammary transplantation capacity. a Single-cell suspensions were prepared from Lgr5KIHET and Lgr5KIHOM littermates at stage E18. Live, single, EpCAMHIGH; CD49fHIGH; GFPPOS cells were sorted and injected into cleared fat pads of recipient mice. Eight weeks later, glands were isolated and carmine stained to visualize outgrowth and take rates were calculated as the fraction of positive outgrowths per number of injections. b Three thousand fMaSCs from Lgr5KIHET and Lgr5KIHOM littermates were transplanted into four fat pads each and outgrowths were produced as described in a. Outgrowths were dissociated and single cells were pooled and measured for GFP, EpCAM, and CD49f expression using FACS. c Four thousand cells from the EpCAMMED; CD49fHIGH population described in Fig. 4b were re-transplanted into two recipient, cleared fat pads for each genotype. Eight weeks later, secondary transplants were carmine stained to visualize epithelium of secondary outgrowths. Images are representatives of three positive outgrowths for each genotype
