Fig. 5
From: Human snoRNA-93 is processed into a microRNA-like RNA that promotes breast cancer cell invasion
Pipox is an endogenous sdRNA-93 target. a Alignment of the putative target site in the Pipox 3′UTR with sdRNA-93. b Pipox LR is specifically repressed by sdRNA-93 in HEK293 transient transfections. Luciferase assays (n = 4) of HEK293 lysates after cotransfection of Pipox LR, Ctl LR, Anti-93, Anti-Ctl, Mimic-93 and / or Mimic-Ctl as indicated (+ or −). Transfections were normalized to respective LR alone. Pipox LR, luciferase reporter containing sdRNA-93 target site from Pipox 3′UTR; Ctl LR, luciferase reporter containing scrambled sdRNA-93 3′UTR target sites; Anti-93, sdRNA-93 inhibitor; Anti-Ctl, control inhibitor; Mimic-93, sdRNA-93 mimic; Mimic-Ctl, control mimic; RLU, relative light units. c Representative western blots of MDA-MB-231 (left) and MCF-7 (right) breast cancer cells transfected with sdRNA-93 inhibitor, sdRNA mimic or scrambled control. Pipox and Vinculin (control) blots are shown. Graphs indicate the relative ratio of Pipox to Vinculin as normalized to nontransfected control. (n ≥ 3)
