Fig. 3
From: PTK6 regulates growth and survival of endocrine therapy-resistant ER+ breast cancer cells
PTK6 downregulation suppresses growth of endocrine therapy-sensitive and resistant ER+ breast cancer cells. MCF-7-EDR, T47D-EDR, or MCF-7-FulvR cells infected with control shRNA (EV) virus or either of two PTK6 shRNA (C9 and 49) viruses were seeded in monolayer cultures (a) or 3D MatrigelTM cultures (b). Cells were re-fed with fresh growth media every 3–4 days. The cells in the 3D cultures were visualized on day 10. Lysates from the PTK6 shRNA-infected cells were probed with the indicated antibodies. Scale bar: 30 μm. All experiments were performed three times. All the western blot images were processed in parallel
