Fig. 1

Fusion genes involving HMGA2 or PLAG1 and somatic mutations targeting HRAS, PIK3CA, AKT1 and PIK3R1 in breast adenomyoepitheliomas. a Heatmap depicting fusion gene and somatic mutations targeting HRAS Q61, PIK3CA and AKT E17 hotspot loci and PIK3R1 mutations identified in breast adenomyoepitheliomas (AMEs; n = 13). Cases are shown in columns and genes in rows. Hotspot mutations are annotated as per Chang et al.²³ b Representative Sanger sequencing electropherograms of HRAS Q61 and PIK3CA hotspot loci in AM16. c Representative hematoxylin and eosin micrographs of an AME harboring an HMGA2-WIF1 fusion gene (AM16), and micrographs depicting p63 and estrogen receptor expression. Scale bars, 500 μm (upper left), 100 μm (upper right) and 50 μm (middle and lower panels). ER estrogen receptor, FISH fluorescence in situ hybridization, SNV single nucleotide variant, WT wild-type