Fig. 2

HMGA2-WIF1 fusion gene identified in the epithelial and myoepithelial cells of a breast adenomyoepithelioma. a Schematic representation of the HMGA2-WIF1 fusion transcript identified in AM16, including the exons and domains involved. HMGA2 is on the (+) DNA strand and WIF1 on the (−) DNA strand. The breakpoints of the 5’ and 3’ partner genes are represented as black vertical lines. Eight spanning reads were found to cross the genomic breakpoint of the HMGA2-WIF1 chimeric transcript and are depicted aligned to the predicted junction sequence. b Representative hematoxylin and eosin and FISH micrographs of the epithelial and myoepithelial components of AM16 using HMGA2 dual-color break apart probes (red, 5′ HMGA2; green, 3′ HMGA2). aa aminoacid, AcD acidic domain, DBD DNA binding domain, E epithelium, M myoepithelium, SpD spacer domain. Scale bar, 50 μm