Fig. 5
NDRG4 knockdown promotes clustering of β1-integrin at cell surface of MCF-7 adherent cells. a Representative of flow citometry histograms using anti-β1 integrin monoclonal antibody (MAB1965) and irrelevant IgG as negative control (IRR). Quantification of the flow cytometry analysis of β1 integrin expression levels at the cell surface of nonadherent MCF-7 shNDRG4 or shSCR cells. Error bars represent SEM of biological replicates (n = 3). *p < 0.01, **p < 0.01, ***p < 0.001, ns = not significant by one-way ANOVA (insert). b Confocal microscopy images of β1 integrin subunit (MAB1965, red) at the surface of VN-adherent MCF-7 shNDRG4 or shSCR cells. c Western blot analysis of β1 integrin subunits and E-cadherin (as loading control) from MCF-7 control (shSCR) or NDRG4-depleted cells (shNDRG4). d Confocal images at the level of the ventral cell surface of individual MCF-7 shNDRG4 (n = 17) or shSCR (n = 20) cells showing different levels of β1 integrin clustering at cell-matrix interface. e Quantification of images in (d) showing the percentage of ventral cell surface covered by clusters of b1 integrin. Each dot represents an individual cell. ***p < 0.001 by two-tailed t tests
