Fig. 2: Preparation of HER2-pulsed dendritic cells vaccines. | npj Breast Cancer

Fig. 2: Preparation of HER2-pulsed dendritic cells vaccines.

From: Clinical development of immunotherapies for HER2+ breast cancer: a review of HER2-directed monoclonal antibodies and beyond

Fig. 2

Preparation of the vaccine is a multi-step process starting with leukapheresis and countercurrent centrifugal elutriation to obtain the relevant monocyte pool. This monocyte pool is then treated with GM-CSF and IL-4, then pulsed with HER peptides and matured with IFN-γ and LPS for conversion into type 1 dendritic cells.

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