Table 1 119 significantly differentially expressed proteins between reactive (n = 5) and metastatic (n = 4) perfusate.
From: Proteomics of REPLICANT perfusate detects changes in the metastatic lymph node microenvironment
Uniprot ID | Description | # Unique peptides | ReactiveAVE | MetastaticAVE | log2ratio | T-test |
|---|---|---|---|---|---|---|
P02655 | Apolipoprotein C-II | 5 | 153.72 | 32.83 | −2.23 | 0.03 |
P21980 | Protein-glutamine gamma-glutamyltransferase 2 | 11 | 144.44 | 44.45 | −1.70 | 0.02 |
P00568 | Adenylate kinase isoenzyme 1 | 8 | 141.80 | 47.70 | −1.57 | 0.04 |
P68036 | Ubiquitin-conjugating enzyme E2 L3 | 2 | 141.16 | 48.55 | −1.54 | 0.01 |
A1L0T0 | Acetolactate synthase-like protein | 2 | 141.06 | 48.73 | −1.53 | 0.01 |
Q9BXN1 | Asporin | 3 | 140.36 | 49.55 | −1.50 | 0.001 |
Q13404 | Ubiquitin-conjugating enzyme E2 variant 1 | 2 | 139.68 | 50.43 | −1.47 | 0.05 |
P51888 | Prolargin | 5 | 139.20 | 50.98 | −1.45 | 0.005 |
P02656 | Apolipoprotein C-III | 2 | 139.16 | 51.00 | −1.45 | 0.03 |
P35611 | Alpha-adducin | 11 | 138.52 | 51.85 | −1.42 | 0.04 |
P40227 | T-complex protein 1 subunit zeta | 5 | 137.98 | 52.58 | −1.39 | 0.05 |
O00159 | Unconventional myosin-Ic | 13 | 136.70 | 54.08 | −1.34 | 0.03 |
Q05469 | Hormone-sensitive lipase | 12 | 136.52 | 54.33 | −1.33 | 0.01 |
P16671 | Platelet glycoprotein 4 | 6 | 136.50 | 54.35 | −1.33 | 0.02 |
P55084 | Trifunctional enzyme subunit beta, mitochondrial | 5 | 136.20 | 54.70 | −1.32 | 0.02 |
O75955 | Flotillin-1 | 4 | 135.58 | 55.58 | −1.29 | 0.04 |
P35232 | Prohibitin | 2 | 159.43 | 65.55 | −1.28 | 0.004 |
P50990 | T-complex protein 1 subunit theta | 11 | 134.98 | 56.30 | −1.26 | 0.03 |
P52943 | Cysteine-rich protein 2 | 3 | 134.94 | 56.38 | −1.26 | 0.03 |
P48643 | T-complex protein 1 subunit epsilon | 9 | 134.48 | 56.88 | −1.24 | 0.01 |
Q8WUM4 | Programmed cell death 6-interacting protein | 9 | 134.40 | 56.98 | −1.24 | 0.01 |
O00151 | PDZ and LIM domain protein 1 | 8 | 134.34 | 57.08 | −1.23 | 0.03 |
P17987 | T-complex protein 1 subunit alpha | 11 | 134.24 | 57.20 | −1.23 | 0.03 |
O75947 | ATP synthase subunit d, mitochondrial | 3 | 133.98 | 57.53 | −1.22 | 0.04 |
Q9NQ79 | Cartilage acidic protein 1 | 3 | 133.82 | 57.73 | −1.21 | 0.03 |
Q02750 | Dual specificity mitogen-activated protein kinase kinase 1 | 3 | 133.34 | 58.33 | −1.19 | 0.003 |
P05091 | Aldehyde dehydrogenase, mitochondrial | 11 | 133.26 | 58.40 | −1.19 | 0.02 |
P38606 | V-type proton ATPase catalytic subunit A | 3 | 133.14 | 58.60 | −1.18 | 0.02 |
Q13200 | 26S proteasome non-ATPase regulatory subunit 2 | 6 | 132.62 | 59.20 | −1.16 | 0.05 |
Q9NVD7 | Alpha-parvin | 6 | 131.72 | 60.35 | −1.13 | 0.05 |
P61088 | Ubiquitin-conjugating enzyme E2 N | 5 | 131.66 | 60.43 | −1.12 | 0.05 |
Q9NZN4 | EH domain-containing protein 2 | 19 | 131.20 | 60.98 | −1.11 | 0.04 |
P35998 | 26S proteasome regulatory subunit 7 | 4 | 130.98 | 61.25 | −1.10 | 0.02 |
O75915 | PRA1 family protein 3 | 4 | 130.18 | 62.33 | −1.06 | 0.04 |
O95747 | Serine/threonine-protein kinase OSR1 | 5 | 130.04 | 62.43 | −1.06 | 0.04 |
P28289 | Tropomodulin-1 | 10 | 130.02 | 62.48 | −1.06 | 0.03 |
Q969G5 | Caveolae-associated protein 3 | 5 | 129.74 | 62.83 | −1.05 | 0.05 |
P16152 | Carbonyl reductase [NADPH] 1 | 5 | 129.52 | 63.08 | −1.04 | 0.05 |
Q9UNH7 | Sorting nexin-6 | 2 | 129.18 | 63.53 | −1.02 | 0.04 |
P11171 | Protein 4.1 | 19 | 129.16 | 63.53 | −1.02 | 0.05 |
P30626 | Sorcin | 7 | 128.06 | 64.95 | −0.98 | 0.04 |
P26447 | Protein S100-A4 | 3 | 127.96 | 65.05 | −0.98 | 0.03 |
P04083 | Annexin A1 | 15 | 127.46 | 65.68 | −0.96 | 0.04 |
P48059 | LIM and senescent cell antigen-like-containing domain protein 1 | 3 | 127.00 | 66.25 | −0.94 | 0.02 |
Q04446 | 1,4-alpha-glucan-branching enzyme | 3 | 125.16 | 68.53 | −0.87 | 0.05 |
P36969 | Phospholipid hydroperoxide glutathione peroxidase | 2 | 124.38 | 69.50 | −0.84 | 0.05 |
P35241 | Radixin | 9 | 123.96 | 70.05 | −0.82 | 0.02 |
P05546 | Heparin cofactor 2 | 10 | 123.84 | 70.20 | −0.82 | 0.05 |
Q8TAT6 | Nuclear protein localisation protein 4 homologue | 2 | 123.80 | 70.28 | −0.82 | 0.05 |
Q9H4A3 | Serine/threonine-protein kinase WNK1 | 5 | 122.78 | 71.55 | −0.78 | 0.03 |
P60660 | Myosin light polypeptide 6 | 7 | 122.56 | 71.83 | −0.77 | 0.04 |
P08697 | Alpha-2-antiplasmin | 6 | 122.54 | 71.88 | −0.77 | 0.05 |
Q13011 | Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial | 3 | 122.16 | 72.33 | −0.76 | 0.03 |
O00233 | 26S proteasome non-ATPase regulatory subunit 9 | 5 | 121.20 | 73.50 | −0.72 | 0.03 |
P13671 | Complement component C6 | 17 | 119.66 | 75.40 | −0.67 | 0.04 |
P00734 | Prothrombin | 23 | 117.14 | 78.60 | −0.58 | 0.04 |
O94903 | Pyridoxal phosphate homoeostasis protein | 2 | 115.56 | 80.53 | −0.52 | 0.004 |
P63244 | Receptor of activated protein C kinase 1 | 8 | 83.76 | 120.30 | 0.52 | 0.01 |
P43034 | Platelet-activating factor acetylhydrolase IB subunit alpha | 11 | 83.36 | 120.80 | 0.54 | 0.05 |
P48147 | Prolyl endopeptidase | 7 | 80.98 | 123.78 | 0.61 | 0.05 |
Q99715 | Collagen alpha-1(XII) chain | 2 | 78.12 | 127.43 | 0.71 | 0.05 |
A1L4H1 | Soluble scavenger receptor cysteine-rich domain-containing protein SSC5D | 6 | 77.54 | 128.10 | 0.72 | 0.04 |
Q03154 | Aminoacylase-1 | 5 | 77.18 | 128.53 | 0.74 | 0.05 |
P62906 | 60S ribosomal protein L10a | 5 | 75.50 | 130.63 | 0.79 | 0.02 |
P02794 | Ferritin heavy chain | 7 | 72.98 | 133.78 | 0.87 | 0.02 |
Q15424 | Scaffold attachment factor B1 | 5 | 72.98 | 133.80 | 0.87 | 0.05 |
P34096 | Ribonuclease 4 | 2 | 72.52 | 134.35 | 0.89 | 0.04 |
P10155 | 60 kDa SS-A/Ro ribonucleoprotein | 11 | 72.36 | 134.55 | 0.89 | 0.04 |
P60900 | Proteasome subunit alpha type-6 | 12 | 71.68 | 135.45 | 0.92 | 0.05 |
Q9P258 | Protein RCC2 | 2 | 70.92 | 136.35 | 0.94 | 0.04 |
P01833 | Polymeric immunoglobulin receptor | 4 | 70.64 | 136.73 | 0.95 | 0.006 |
Q9BRA2 | Thioredoxin domain-containing protein 17 | 3 | 70.62 | 136.70 | 0.95 | 0.05 |
P55786 | Puromycin-sensitive aminopeptidase | 25 | 69.06 | 138.65 | 1.01 | 0.05 |
Q9Y646 | Carboxypeptidase Q | 3 | 68.80 | 139.00 | 1.01 | 0.02 |
Q12765 | Secernin-1 | 6 | 68.36 | 139.53 | 1.03 | 0.03 |
Q08380 | Galectin-3-binding protein | 10 | 66.60 | 141.70 | 1.09 | 0.002 |
Q96KP4 | Cytosolic non-specific dipeptidase | 19 | 65.62 | 142.98 | 1.12 | 0.05 |
Q9BTY2 | Plasma alpha-L-fucosidase | 3 | 65.56 | 143.08 | 1.13 | 0.05 |
Q9BY67 | Cell adhesion molecule 1 | 2 | 65.24 | 143.45 | 1.14 | 0.03 |
Q9Y279 | V-set and immunoglobulin domain-containing protein 4 | 2 | 64.62 | 144.28 | 1.16 | 0.03 |
P02792 | Ferritin light chain | 8 | 64.00 | 145.00 | 1.18 | 0.03 |
P02790 | Hemopexin | 29 | 63.04 | 146.23 | 1.21 | 0.05 |
O94760 | N(G),N(G)-dimethylarginine dimethylaminohydrolase 1 | 6 | 62.68 | 146.63 | 1.23 | 0.04 |
Q15848 | Adiponectin | 2 | 62.36 | 147.03 | 1.24 | 0.04 |
P16083 | Ribosyldihydronicotinamide dehydrogenase [quinone] | 6 | 61.62 | 147.98 | 1.26 | 0.04 |
P29401 | Transketolase | 28 | 61.58 | 148.03 | 1.27 | 0.03 |
Q14126 | Desmoglein-2 | 6 | 61.34 | 148.35 | 1.27 | 0.0006 |
O75368 | SH3 domain-binding glutamic acid-rich-like protein | 9 | 60.98 | 148.78 | 1.29 | 0.04 |
Q92820 | Gamma-glutamyl hydrolase | 6 | 60.32 | 149.55 | 1.31 | 0.04 |
Q8NCW5 | NAD(P)H-hydrate epimerase | 5 | 59.88 | 150.18 | 1.33 | 0.04 |
P06748 | Nucleophosmin | 3 | 59.52 | 150.63 | 1.34 | 0.02 |
P40394 | Alcohol dehydrogenase class 4 mu/sigma chain | 2 | 57.40 | 153.28 | 1.42 | 0.05 |
Q86VB7 | Scavenger receptor cysteine-rich type 1 protein M130 | 17 | 56.38 | 154.55 | 1.45 | 0.007 |
P35527 | Keratin, type I cytoskeletal 9 | 21 | 54.94 | 156.30 | 1.51 | 0.04 |
P07686 | Beta-hexosaminidase subunit beta | 7 | 54.56 | 156.78 | 1.52 | 0.01 |
P34059 | N-acetylgalactosamine-6-sulfatase | 3 | 54.30 | 157.13 | 1.53 | 0.04 |
P28838 | Cytosol aminopeptidase | 20 | 52.70 | 159.15 | 1.59 | 0.03 |
P06865 | Beta-hexosaminidase subunit alpha | 5 | 52.08 | 159.88 | 1.62 | 0.02 |
P06454 | Prothymosin alpha | 2 | 50.02 | 162.48 | 1.70 | 0.005 |
P00738 | Haptoglobin | 14 | 49.14 | 163.58 | 1.73 | 0.05 |
P04264 | Keratin, type II cytoskeletal 1 | 30 | 48.58 | 164.30 | 1.76 | 0.02 |
P08637 | Low affinity immunoglobulin gamma Fc region receptor III-A | 4 | 48.32 | 164.60 | 1.77 | 0.03 |
P25311 | Zinc-alpha-2-glycoprotein | 19 | 46.80 | 166.50 | 1.83 | 0.05 |
P20908 | Collagen alpha-1(V) chain | 3 | 46.76 | 166.50 | 1.83 | 0.02 |
P27695 | DNA-(apurinic or apyrimidinic site) lyase | 10 | 44.50 | 169.33 | 1.93 | 0.05 |
P35908 | Keratin, type II cytoskeletal 2 epidermal | 22 | 44.34 | 169.58 | 1.94 | 0.02 |
Q99729 | Heterogeneous nuclear ribonucleoprotein A/B | 2 | 44.24 | 169.70 | 1.94 | 0.05 |
P28065 | Proteasome subunit beta type-9 | 3 | 43.40 | 170.70 | 1.98 | 0.01 |
Q8N1N4 | Keratin, type II cytoskeletal 78 | 2 | 42.74 | 171.58 | 2.01 | 0.03 |
Q96C23 | Aldose 1-epimerase | 7 | 42.46 | 171.93 | 2.02 | 0.02 |
P13645 | Keratin, type I cytoskeletal 10 | 24 | 41.14 | 173.58 | 2.08 | 0.01 |
Q5D862 | Filaggrin-2 | 3 | 41.00 | 173.75 | 2.08 | 0.0004 |
P02750 | Leucine-rich alpha-2-glycoprotein | 6 | 40.84 | 173.95 | 2.09 | 0.02 |
P40306 | Proteasome subunit beta type-10 | 3 | 39.42 | 175.73 | 2.16 | 0.02 |
Q9NZK5 | Adenosine deaminase 2 | 5 | 36.10 | 179.85 | 2.32 | 0.03 |
P09467 | Fructose-1,6-bisphosphatase 1 | 9 | 35.96 | 180.05 | 2.32 | 0.03 |
P21741 | Midkine | 2 | 35.18 | 181.03 | 2.36 | 0.04 |
Q13740 | CD166 antigen | 8 | 34.90 | 181.40 | 2.38 | 0.005 |
P12830 | Cadherin-1 | 8 | 19.48 | 200.65 | 3.36 | 0.01 |
