Fig. 1: MCF10AT hPRLrL + I co-expression in vivo.
From: The human intermediate prolactin receptor is a mammary proto-oncogene
a MCF10AT cells were stably transfected with either hPRLrL, hPRLrI, both isoforms together (at a 1:1 ratio), or empty vector (MCF10AT-hPRLrL, MCF10AT-hPRLrI, MCF10AT-hPRLrL+I, and MCF10AT-EV, respectively), and expression was confirmed by IB. b These cells were orthotopically xenografted into female NSG mice, and primary tumor growth was monitored longitudinally c Lungs were harvested and micrometastatic burden was counted manually. Representative images (scale bar, 100 µm) of H&E stained d primary tumor, e axillary lymph node and f lung indicate primary tumors to be high grade adenocarcinoma with nodal infiltration and successful distant metastatic dissemination (outlined; representative image shows metastatic growth long a blood vessel). *p < 0.05, **p < 0.01, ***p < 0.005. n = 4 mice/study arm. Data is shown as mean ± SEM.
