Fig. 3: In vitro knock-down of hPRLrI in MCF7 cells.
From: The human intermediate prolactin receptor is a mammary proto-oncogene
a MCF7 cells were stably transfected with either scrambled RNA (scRNA) negative control or shRNA targeting the hPRLrI splice site. These transfectants were analyzed for differential anchorage-independent growth via soft agar assay, quantifying both b colony number and c colony size, using CellProfiler. Transfectants were further assessed for d proliferative potential and e ability to migrate. *p < 0.05, ****p < 0.001, n = 3. Data is shown as mean ± SEM.
