Fig. 4: hPRLr heterodimer versus homodimer differential stability.
From: The human intermediate prolactin receptor is a mammary proto-oncogene
Chinese hamster ovary (CHO) cells (PRLr-null) were transiently-transfected with either hPRLrL, hPRLrI, or both isoforms concurrently (at a 1:1 ratio). a Transfectants were simultaneously stimulated with cycloheximide (CHX; 100 µg/mL) and prolactin (PRL; 250 ng/mL), and lysates were harvested once per hour for 5 consecutive hours. Protein degradation was quantified, via densitometry of band intensity, in b and t1/2 was extrapolated from each respective slope. c Phosphorylation status of the hPRLr phosphodegron was assessed using a pS349-specific antibody (gift of Dr. Serge Fuchs), and d band intensity was quantified via densitometry. *p < 0.05, **p < 0.01, ***p < 0.005, n = 3. Data is shown as mean ± SEM.
