Fig. 7: Protein and transcript expression of hPRLrI in breast cancer clinical samples.
From: The human intermediate prolactin receptor is a mammary proto-oncogene
hPRLrI protein in breast cancer clinical samples (n = 250) was analyzed by tissue microarray (TMA) analysis. Allred score is reflective of both hPRLrI staining intensity and percent positivity. a Proliferative index, reported via Ki67 status, was assessed, as was b tumor grade and c hormone receptor status. d Proliferative index within the ER-/PR-/Her2- cohort was also analyzed. The hPRLrI:hPRLrL transcript ratio was also examined, using TCGA-reposited RNAseq data. e Breast cancer intrinsic subtype frequency was assessed for the hPRLrIhi/hPRLrLlo versus hPRLrIlo/hPRLrLhi ratio tertiles (n = 67/tertile). f Differential gene expression patterns were characterized, with the heatmap displaying the top/bottom 50 differentially-expressed genes (DEGs), comparing the top/bottom hPRLr isoform ratio tertile. These DEGs were then analyzed via GSEA, looking within the g chemical and genetic perturbation and h oncogenic signatures gene sets. *p < 0.05, **p < 0.01, ***p < 0.005, ****p < 0.001. Data is shown as mean ± SEM.
