Fig. 7: GnRHa relieves ER stress by inhibiting mTOR pathway to induce autophagy in CTX-treated mice. | npj Breast Cancer

Fig. 7: GnRHa relieves ER stress by inhibiting mTOR pathway to induce autophagy in CTX-treated mice.

From: GnRHa protects the ovarian reserve by reducing endoplasmic reticulum stress during cyclophosphamide-based chemotherapy

Fig. 7

To explore the effect of CTX and/or GnRHa on mouse ovaries, 8-week-old tumor-bearing nude mice were treated with saline, 200 mg/kg CTX, and 200 mg/kg CTX + GnRHa, and ovarian protein were extracted 1 week after treatment. a Expression of proteins in the ER stress pathway was assessed by western blotting. b Expressions of proteins in the autophagy were assessed by western blotting. c Expressions of proteins in the mTOR pathway were assessed by western blotting. d The GRP78, ATF4, XBP1, and CHOP increased significantly in CTX-treated ovaries than the control or CTX + GnRHa group. e Compared with CTX-treated mice, LC3B and Beclin-1 were increased, and P62 was decreased in coadministration with GnRHa mice. f, g The phosphorylation of mTOR and P70S6K were significantly increased in ovaries of CTX alone treated mice. Data are expressed as mean ± SD of three independent experiments. All error bars represent the standard error of the mean. Two-tailed Student’s t test was used for statistical analysis, *P < 0.05, **P < 0.001, and ***P < 0.0001.

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