Fig. 4: MMP13 expression correlates with human DCIS progression to invasion and promotes breast cancer cell invasion in vitro. | npj Breast Cancer

Fig. 4: MMP13 expression correlates with human DCIS progression to invasion and promotes breast cancer cell invasion in vitro.

From: Mechanostimulation of breast myoepithelial cells induces functional changes associated with DCIS progression to invasion

Fig. 4

a Representative images of MMP13 by immunohistochemical staining in human breast tissue samples featuring adjacent normal ducts, DCIS and DCIS/IDC. Scale bar, 20 μm. b Bar graphs showing the mean and individual percentage of ducts positive for MMP13 in adjacent normal (n = 40), DCIS (n = 20) and DCIS/IDC (n = 20) patient samples (error bars, +s.e.m). ****P < 0.0001 (Kruskal–Wallis one-way ANOVA). c mRNA expression of MMP13 in normal breast tissues (n = 5), DCIS (n = 9) and IDC (n = 5) (error bars, +s.e.m). * P = 0.0373 (Kruskal–Wallis one-way ANOVA). d–g Bar graphs showing qRT-PCR analysis for MMP13 using RNA isolated from DCIS myoepithelial cells with integrin β6-low and β6-high expression (d), myoepithelial cell lines; N-1089 and β6-1089 (e) and from primary normal myoepithelial cells; 1989 (f) and 1492 (g) transfected with an empty vector (N-) or integrin β6 expression construct (β6-) (error bars, +s.e.m). n = 3 biological replicates, 3 technical replicates; ****P < 0.0001 (d,e), ***P = 0.0001 (f) and ***P = 0.0006 (g) (two-tailed t-test). h Invasion assay for MDA-MB-231 (231) and MCF-7 cells using conditioned media (CM) isolated from myoepithelial cell line; β6-1089 transfected with a control (CTL) or MMP13-targeted (MMP13KD) siRNA (error bars, +s.e.m). n = 3 biological replicates, 4-6 technical replicates; ***P = 0.0001 (two-tailed t-test).

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