Fig. 6: Mechanical stretching normal myoepithelial cells activates a tumour promoting phenotype.

a Invasion assay for MDA-MB-231 (231) and MCF-7 cells using conditioned media (CM) isolated from unstretched (Unstr) or stretched (Str) normal myoepithelial cell line; N-1089 (error bars, +s.e.m). n = 3 biological replicates, 5 technical replicates; ****P < 0.0001 (two-tailed t-test). b Array analysis for proteases using conditioned media isolated from unstretched or stretched myoepithelial cell line; N-1089 (error bars, +s.e.m). n = 1 biological replicate, 2 technical replicates. c–e Gelatin zymography for MMP9 activity using conditioned media isolated from unstretched or stretched myoepithelial cell line; N-1089 (c) and primary normal myoepithelial cells; N-1989 (d) and N-1492 (e). Images are representative of three experiments. f–h Bar graphs showing qRT-PCR analysis for MMP9 using RNA isolated from unstretched or stretched myoepithelial cell line; N-1089 (f) and primary normal myoepithelial cells; N-1989 (g) and N-1492 (h) (error bars, +s.e.m). n = 3 biological replicates, 3 technical replicates; **P = 0.0035 (f), ***P = 0.0005 (g) and *P = 0.0232 (h) (two-tailed t-test). i–k Bar graphs showing qRT-PCR analysis for MMP13 using RNA isolated from unstretched or stretched myoepithelial cell line; N-1089 (i) and primary normal myoepithelial cells; N-1989 (j) and N-1492 (k) (error bars, +s.e.m). n = 3 biological replicates, 3 technical replicates; **P = 0.0017 (i), ***P = 0.0007 (j) and ***P = 0.0009 (k) (two-tailed t-test).