Fig. 5: β6 drives TGFβ dependent activation of EP300, which in turn regulates MMP13 expression.
From: TGFβ-mediated MMP13 secretion drives myoepithelial cell dependent breast cancer progression
a Predicted activation Z-scores of TGFβ dependent β6-driven transcriptional regulators, based on expression changes of target genes in 1089iβ6 cells following β6 induction. b Relative MMP13 mRNA expression following siRNA knock down of transcriptional regulators. NTC non-targeting control. Fold change <2 indicates positive regulators of MMP13 (red) and fold change >5 indicates negative regulators of MMP13 (blue). c Relative MMP13 mRNA expression in 1089iβ6 cells transfected with NTC or EP300 siRNA, treated with doxycycline (1 µg/mL) for 48 h. d Relative MMP13 mRNA expression in 1089iβ6 cells treated with doxycycline (1 µg/mL) and/or SGCCBP30 (1 µM) for 48 h. e ChIP-qPCR analysis for enrichment of H3K27ac at the MMP13 promoter region in 1089iβ6 cells following treatment with doxycycline (1 µg/mL) and/or SGCCBP30 (1 µM) for 48 h. *p < 0.05, ***p < 0.001 (One-way ANOVA with multiple comparisons). Data are presented as mean ± SEM with biological replicates indicated by different colours. Data are representative of three independent experiments.
