Fig. 6: β6 integrin-driven invasion is EP300/MMP13 dependent in a physiologically relevant ductal model of DCIS.
From: TGFβ-mediated MMP13 secretion drives myoepithelial cell dependent breast cancer progression
Primary luminal and myoepithelial cell ductal structures are formed 14 days after embedding into collagen gels and subsequently treated with doxycycline (1 µg/mL) for a further 7 days to induce transgene expression. To inhibit activity of MMP13 and EP300, ducts were treated with MMP13i (CAS-544678 85-5 1 µM) and EP300i (SGCCBP30 1 µM), respectively. a Representative light micrographs of ductal structure at 14 days post embedding. b Imaris reconstructions of luminal/myoepithelial ductal structures after 21 days of culture with HER2 luminal expression −/+ myoepithelial β6 expression and inhibition of MMP13 or EP300 where Nuclei (blue), Vimentin (magenta), HER2 (green) and Cleaved Collagen (yellow). Scale bar = 20 µm.
