Fig. 3: CXorf56 knockdown promotes the accumulation of DNA damage in TNBC cells upon IR treatment.
From: Inhibition of CXorf56 promotes PARP inhibitor-induced cytotoxicity in triple-negative breast cancer
CXorf56 stable knockdown efficiency was detected by qPCR in TNBC cells including MDA-MB-231 (A), BT549 (B), and SUM1315 (C) cells, respectively. Data were analyzed by a two-tailed t test. Negative control (NTC) and CXorf56-knockdown TNBC cells (D, E, MDA-MB-231; F, G, BT549; H, I, SUM1315) were treated without (Ctrl) or with IR (2 Gy), γ-H2AX foci before or 8 hours after IR was detected by immunofluorescence. Nuclei were visualized with DAPI (blue). Representative images of γ-H2AX foci are shown in D, F, H. Quantification of focus signals is shown in E, G, I. Data were analyzed by ANOVA and two-tailed t test. Remarks: *p < 0.05, **p < 0.01, ***p < 0.001; Data are presented as mean ± SD. Scale bars = 10 μm.
