Table 2 Patient and tumor characteristics at the time of first RS and second RS testing in the study cohort.

	First BC n = 60	Second BC n = 60	p*
Median (IQR) age at testing, years	55.5 (44–62)	60.5 (50–67)	<0.001
Median (IQR) tumor size, cm	1.6 (1.1–2.2)	1.4 (1.0–1.8)	0.54
Tumor size category, n (%)			0.096^a
≤1 cm	15 (25.0%)	16 (26.7%)
>1-2 cm	27 (45.0%)	32 (53.3%)
>2 cm	18 (30.0%)	9 (15.0%)
Not available	0 (0%)	3 (5.0)
Tumor grade category, n (%)			0.79^b
Grade 1	9 (15.0%)	6 (10.0%)
Grade 2	32 (53.3%)	36 (60.0%)
Grade 3	10 (16.7%)	9 (15.0%)
Not available	9 (15.0%)	9 (15.0%)
Histology, n (%)			0.75^c
IDC	50 (83.3%)	48 (80.0%)
ILC	5 (8.3%)	10 (16.7%)
Mucinous/colloid/papillary	2 (3.3%)	1 (1.7%)
Other/not available	3 (5.0%)	1 (1.7%)
Nodal status, n (%)			0.21^d
N0	46 (76.7%)	49 (81.7%)
N1mi	8 (13.3%)	1 (1.7%)
N1	6 (10.0%)	7 (11.7%)
Not available	0 (0.0%)	3 (5.0%)

BC breast cancer, IDC invasive ductal carcinoma, ILC invasive lobular carcinoma, IQR interquartile range.
*p value for Wilcoxon signed-rank test and the McNemar’s test for continuous and categorical variables, respectively. Bolded p values are statistically significant. McNemar’s test contingency tables are presented in Supplementary Table 1.
^aFor comparing tumor size ≥2 cm to <2 cm.
^bFor comparing grade 1/2 to grade 3.
^cFor comparing IDC to non-IDC histologies.
^dFor comparing N0 to N1/N1mi patients.

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