Table 4 Qualified antibody staining panel.
Cycle # | |||
|---|---|---|---|
Background | DAPI_1 | ||
FITC_1 | A488 | Background | |
Cy3_1 | A555 | Background | |
Cy5_1 | A647 | Background | |
2 | DAPI_2 | ||
FITC_2 | HER2 (TF-MA5-14509) | Thermo (Rabbit) | |
Cy3_2 | 53BP1 (Bethy A303-906A) | Bethyl (Goat) | |
Cy5_2 | p53 (Dako_M7001) | DAKO (DO-7 IgG2b) | |
3 | DAPI_3 | ||
FITC_3 | PR-488 (ab199244) | Abcam | |
Cy3_3 | ER-PE (CS74244) | CST | |
Cy5_3 | PR-647 (ab199455) | Abcam | |
4 | DAPI_4 | ||
FITC_4 | Ki67-488 (CS11882) | CST | |
Cy3_4 | HER2-PE (CS98710) | CST | |
Cy5_4 | AR-647 (AB194195) | Abcam | |
5 | DAPI_5 | ||
FITC_5 | CD45-488 (FAB1430G) | R&D (2D1 clone) | |
Cy3_5 | CK-570 (EB41-9003-82) | Ebio | |
Cy5_5 | p53-647 (ab224942) | Abcam | |
6 | DAPI_6 | ||
FITC_6 | p53-FITC (Bio645803) | Biolegend (DO-7) | |
Cy3_6 | PD-L1-555 (AB206616) | Abcam | |
Cy5_6 | HER2-647 (ab225510) | Abcam | |
7 | DAPI_7 | ||
FITC_7 | CD68-488(CST24850) | CST | |
Cy3_7 | pRb-555(CST8957) | CST | |
Cy5_7 | PD-L1-647(CST15005) | CST |
