Fig. 1: Mass cytometry analysis of major immune subsets in peripheral blood of breast cancer (BC) patients and healthy donors (HD). | npj Breast Cancer

Fig. 1: Mass cytometry analysis of major immune subsets in peripheral blood of breast cancer (BC) patients and healthy donors (HD).

From: Peripheral immune cells in metastatic breast cancer patients display a systemic immunosuppressed signature consistent with chronic inflammation

Fig. 1

a UMAP analysis of PBMCs using 12 phenotypic markers (CD3, CD4, CD8, TCRgd, CD19, CD56, CD16, CD33, CD14, CD11c, HLA-DR, and CD303). Different immune subsets were identified by phenograph clustering. b Heat map depicting mean expression of phenotypic markers on different immune subsets. c Abundance of lymphocyte and myeloid immune cell subsets in BC patients (n = 104) and HD (n = 20). d Heat map representing hierarchical clustering of all samples based on abundance of different immune subsets. Groups were compared with Fisher’s exact test. e Abundance of lymphocyte and myeloid cell subsets in patients with HR+ BC (n = 57) or TNBC (n = 47). f Effect of previous therapy on immune cells: Received no chemotherapy/CDK inhibitors (Chemo−CDK−; n = 10), received chemotherapy/CDK inhibitors > 3 months before sample collection [Previous Chemo/CDK (not last 3 months); n = 40], received chemotherapy within 3 months before sample collection [Chemo (last 3 months); n = 26], received CDK inhibitors within 3 months before sample collection [CDK (last 3 months); n = 28]. g Absolute numbers of immune cell subsets per liter blood of HR+ BC (n = 57) and TNBC patients (n = 47). h Absolute numbers of immune cell subsets per liter blood of patients grouped based on previous treatments. Two groups were compared by Wilcoxon Mann–Whitney Rank Sum Test. More than two groups were compared by Kruskal–Wallis test, followed by pair-wise comparisons using Wilcoxon Mann–Whitney Rank Sum Test using HD (Chemo−CDK− for Fig. 1h) as a reference group. FDR-adjusted p-value (p.adj) <0.05 was considered statistically significant. *p.adj < 0.05, **p.adj < 0.01, ***p.adj < 0.001, ****p.adj < 0.0001. Box plots show median (center line), 25th and 75th percentiles (box), data within 1.5 IQR (whiskers), and outliers (dots). Abbreviations: T, T cells, B, B cells, NK Natural Killer cells, mDC myeloid dendritic cells, pDC plasmacytoid dendritic cells, DN double negative, DP double positive.

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