Table 3 Antibody panels for flow cytometric analysis
From: Sustained lymphocyte decreases after treatment for early breast cancer
Surface stain | Fluorophore | Dilution | Supplier/reference number |
|---|---|---|---|
Live/dead | PE Texas Red | 1:800 | Biolegend #423110 |
CD4 | PE | 1:200 | Biolegend #317410 |
CD3 | AF700 | 1:100 | Biolegend #344822 |
CD8 | BV510 | 1:100 | Biolegend #344732 |
CD45RA | BV711 | 1:100 | Biolegend #304138 |
CCR7 | PE-Cy7 | 1:100 | Biolegend #353226 |
PD-1 | BV605 | 1:100 | Biolegend #329924 |
CD31 | APC-Cy7 | 1:100 | BD Bioscience #563653 |
CD25 Intracellular stain | BUV395 | 1:100 | BD Bioscience #564034 |
IFN-gamma | AF488 | 1:100 | Biolegend #502515 |
TNF-alpha | BV785 | 1:100 | Biolegend #502948 |
CTLA-4 | APC | 1:100 | Biolegend #349908 |
FOXP3 | BV421 | 1:50 | Biolegend #320124 |
IL2 | BB700 | 1:100 | BD Bioscienes #566406 |
