Fig. 5: Consensus targets in ESR1 mutant breast cancer cell models.

A A bar plot ranking the genes by consisteny of falling into top10% upregulated targets in ESR1 mutant cell models. Only genes showing equal to or above 50% consistency out of 46 comparisons were shown. Color code indicates the number of cohorts (four in total) in which this gene is also identified as a significantly upregulated target in ESR1 mutant samples. B Scatter plot showing the correlation of average regulatory percentile between ESR1 mutant and estrogen treatment of 25 targets in (A). C Box plots showing the expression of NPY1R between ESR1 WT and mutant samples from the three cohorts. D, E Bar plot showing the distribution of regulatory percentile of NPY1R in all the comparisons from ESR1 mutant cells (D), estrogen and ER modulator treatments (E) experiments. Percentiles are ranged between −100 to +100 and larger number indicates stronger regulation. F Genomic track view of ER ChIP-seq signal from ESR1 WT and ESR1 mutant models in two cell lines from GSE148277. Genomic track scale is adjusted to the identical level for all the samples. G Box plots showing the relative expression of NPY1R in genome-edited MCF7 and T47D ESR1 mutant cells and WT treated with E2 condition. ∆ ∆Ct method is used and p values were calculated using one-way ANOVA. H Line plot showing the growth rate of T47D WT and ESR1 mutant cells in the presence of scramble (siNT) and NPY1R siRNA transfection for 8 days. Two-way ANOVA was used for statistic comparison. Experiment has been repeated for 3 times.