Table 1 Primers employed for qRT-PCR
From: Phenotypic profiling of CFTR modulators in patient-derived respiratory epithelia
Gene | Forward | Reverse |
|---|---|---|
1. CFTR | 5′‐GCATTTGCTGATTGCACAGT‐3′ | 5′‐CTGGATGGAATCGTACTGCC‐3′ |
2. GAPDH | 5′‐CAAGAGCACAAGAGGAAGAGAG‐3′ | 5′‐CTACATGGCAACTGTGAGGAG‐3′ |
3. FOXJ1 | 5′‐GAGCGGCGCTTTCAAGAAG‐3′ | 5′‐GGCCTCGGTATTCACCGTC‐3′ |
4. MRP4 | 5′‐GGACAAAGACAACTGGTGTGCC‐3′ | 5′‐AATGGTTAGCACGGTGCAGTGG‐3′ |
