Fig. 1: MACROD2 is frequently affected by SVs in HCC, and down-regulation of MACROD2 correlates with patient poor prognosis.

a The somatic SV spectrum in 49 HCCs identified by WGS. Genes containing SVs in at least three samples are shown. b Different types of SVs affecting MACROD2 are indicated by lines with different colors (also exhibited in Table 1). c MACROD2 mRNA expression in 49 HCC tumor tissues compared with that in adjacent non-tumor tissues. d Representative MACROD2 staining in peritumor tissues and tumor tissues with no MACROD2 SV and with MACROD2 SV (25T). Scale bars = 100 μm. e The statistics of the MACROD2 staining density among different groups in 49 HCCs involved in WGS. f MACROD2 expression examined by qRT-PCR and western blot in one normal liver cell line (L0-2) and six HCC cell lines. g Representative HCC tumor and peritumor samples in the FFPE cohort showing the expression of MACROD2: patient 1, high MACROD2 expression; patient 2, low MACROD2 expression. Scale bar = 100 μm. h Kaplan–Meier survival analysis showing survival rates and cumulative recurrence rates on the basis of MACROD2 expression in the FFPE cohort. Data are shown as the mean ± standard deviation (SD) and are representative of three independent experiments.