Fig. 2: MACROD2 deficiency promotes cell proliferation, colony formation, migration, and invasion in vitro, and tumor growth and metastasis in vivo. | npj Genomic Medicine

Fig. 2: MACROD2 deficiency promotes cell proliferation, colony formation, migration, and invasion in vitro, and tumor growth and metastasis in vivo.

From: MACROD2 deficiency promotes hepatocellular carcinoma growth and metastasis by activating GSK-3β/β-catenin signaling

Fig. 2

a Proliferation of MACROD2-knockdown HepG2 and PLC/PRF/5 cells and MACROD2-overexpressing HCCLM3 and MHCC97H cells compared with that of control cells. *P < 0.05, **P < 0.01. b Colony formation activity of MACROD2-knockdown HepG2 and PLC/PRF/5 cells and MACROD2-overexpressing HCCLM3 and MHCC97H cells compared with that of control cells. The bar graphs illustrate the quantification of colony formation. **P < 0.01. c Cell monolayers were examined microscopically in wound-healing migration assays at 24 h post wounding. *P < 0.05. d Invasion of MACROD2-knockdown HepG2 and PLC/PRF/5 cells and MACROD2-overexpressing HCCLM3 and MHCC97H cells compared with that of control cells. The graphs depict the number of invasive cells after 48 h. **P < 0.01. e, f Macrograph of tumors and H&E-stained images of metastatic nodules in the selected areas of lungs in all groups. *P < 0.05, **P < 0.01. Data are shown as the mean ± SD and are representative of three independent experiments.

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