Fig. 7: Robustness of the proposed IMS signatures.

A Comparison of AUC performance of IFN-γ/IMS ratio with nine published GEP signatures (Table 1) after 35 bootstrapping randomized tests on the seven datasets (Table 2). In each randomized test, the IMS genes were identified from three randomly selected datasets (see “Methods” section), and the AUC prediction performance of the ratio of IFN-γ signature and the IMS identified from the randomly selected dataset was evaluated on the remaining four datasets. Models are sorted by their median AUC performances and Wilcoxon matched-pairs signed rank test was performed to compare the AUC performances of IFN-γ/IMS and the second best model T eff. B Top 23 highly frequent genes from the bootstrapping tests. Genes in the original IMS signature are marked with an asterisk (*) symbol. C–D t-SNE plot of cells from melanoma⁴². Cells are colored by cell types in C and by normalized expression of different IMS genes in D.