Fig. 3: Temporal trends in hospital costs around the time of rWGS testing.

Evaluation of spending trend surrounding the rWGS process showed an overall decreased spending post rWGS results (Supplementary Table 2b). There is a significant association between time period and cost (p = 0.01; repeated measures ANOVA). Specifically, there is a significant difference in cost between periods 1 and 3 (mean difference 2266.4; 95% CI 1035.6–3497.1; *p = 0.001; paired t-test) and periods 2 and 3 (mean difference 1917.1; 95% CI 1077.2–2756.9; **p = 0.0001; paired t-test). There is not a significant difference in cost between the nondiagnostic and diagnostic groups by time period (p = 0.70; repeated measures ANOVA).