Fig. 3: Consensus calling increases the accuracy of base calling in the backbone.
From: Accurate detection of circulating tumor DNA using nanopore consensus sequencing
a 1 - single-nucleotide false positive (snFP) rate in backbones (BB22, BB24, and BB25) per number of repeats. The dashed line indicates 10 repeats. Colors represent backbone type. b Mean improvement in snFP rate in BB24 if only the forward or the reverse reads are taken into account. The dashed line indicates an improvement of 0.1%. Colors represent read orientation. c Mean snFP rate across BB24 in reads with at least 10 repeats. Reference sequence is depicted below the x-axis. Colors represent base type. N = any. d Percentage of positions in BB24 with indicated snFP percentage. Data points represent individual sequencing runs. 6 BB22, 8 BB24, and 5 BB25 runs were used for the calculations. Error bars indicate the standard deviation (sd).
